Inside the
laboratory.
By the
numbers.
HPLC purity
third-party verifications
maintained · 96-hr transit
verification & shipped
From sequence to
sealed vial.
Solid-phase peptide synthesis
The peptide chain is assembled amino acid by amino acid on a polymer resin using Fmoc chemistry. Coupling efficiency is monitored at every step.
Cleavage & deprotection
Trifluoroacetic acid cleaves the assembled peptide from the resin and removes side-chain protecting groups. The crude peptide is precipitated and dried.
Reverse-phase HPLC
The crude peptide passes through a C18 column with a precision-controlled gradient. Pure fractions are collected; impurities are discarded.
Mass spectrometry
ESI-MS confirms the molecular weight of the purified peptide matches the theoretical mass within 0.1 Daltons.
Lyophilization
Purified peptide is frozen, then sublimated under vacuum to remove all water. The resulting white powder is stable for years at 2-8°C.
Sterile vialing
In our class 100,000 cleanroom, lyophilized peptide is weighed into Type 1 borosilicate vials, capped with butyl rubber stoppers, and crimp-sealed.
Endotoxin & sterility
Each vial undergoes USP <71> sterility testing and a LAL endotoxin assay. Bacterial endotoxin must read < 0.5 EU/mg to clear release.
Internal HPLC verification
A representative sample from every batch is rerun on HPLC by our internal QA team — independent of the synthesis chemist who produced it.
Independent verification
The same batch is shipped to ARC Labs (ISO 17025) for third-party HPLC analysis. Results must agree with our internal data within 0.2% tolerance.
The tools.
Every instrument that touches a Uvie batch, calibrated quarterly, IQ/OQ/PQ documented.
Through the glass.
Schedule a virtual
walk-through.
We host quarterly virtual lab tours for principal investigators and procurement officers. 45 minutes, live with our QA lead, all questions welcome.
Request a tour →