Inside the
laboratory.
From sequence to
sealed vial.
Solid-phase peptide synthesis
The peptide chain is assembled amino acid by amino acid on a polymer resin using Fmoc chemistry. Coupling efficiency is monitored at every step.
Cleavage & deprotection
Trifluoroacetic acid cleaves the assembled peptide from the resin and removes side-chain protecting groups. The crude peptide is precipitated and dried.
Reverse-phase HPLC
The crude peptide passes through a C18 column with a precision-controlled gradient. Pure fractions are collected; impurities are discarded.
Mass spectrometry
ESI-MS confirms the molecular weight of the purified peptide matches the theoretical mass.
Lyophilization
Purified peptide is frozen, then sublimated under vacuum to remove all water. The resulting white powder is stable for years at 2-8°C.
Sterile vialing
Lyophilized peptide is weighed into Type 1 borosilicate vials, capped with butyl rubber stoppers, and crimp-sealed.